Part:BBa_K4721002:Design
PmxaF
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 154
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The range of the PmxaF promoter sequence as decribed by Schada Von Borzyskowski et al. (2015) is only part of the submitted sequence. There is a scar of a lac promoter, which is an artifact from the cloning process. The results were obtained with this scar included, therefore the part is sumbitted including this cloning artefact
Source
PmxaF is the promoter for the mxaF gene in M. extorquens. This gene encodes the large subunit of methanol dehydrogenase1, which is highly expressed under methylotrophic conditions [1]
References
[1] Liu Q, Kirchhoff JR, Faehnle CR, Viola RE, Hudson RA. A rapid method for the purification of methanol dehydrogenase from Methylobacterium extorquens. Protein Expr Purif. 2006;46(2):316-320. doi:10.1016/J.PEP.2005.07.014
[2] Schada Von Borzyskowski L, Remus-Emsermann M, Weishaupt R, Vorholt JA, Erb TJ. A set of versatile brick vectors and promoters for the assembly, expression, and integration of synthetic operons in methylobacterium extorquens am1 and other alphaproteobacteria. ACS Synth Biol. 2015;4(4):430-443. doi:10.1021/sb500221v
[3] Peyraud R, Kiefer P, Christen P, Massou S, Portais JC, Vorholt JA. Demonstration of the ethylmalonyl-CoA pathway by using13C metabolomics. Proc Natl Acad Sci U S A. 2009;106(12):4846-4851. doi:10.1073/pnas.0810932106
[4] Leveau JHJ, Lindow SE. Predictive and Interpretive Simulation of Green Fluorescent Protein Expression in Reporter Bacteria. J Bacteriol. 2001;183(23):6752-6762. doi:10.1128/JB.183.23.6752-6762.2001